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Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases†

机译：CYP153家族的细胞色素P450烷烃羟化酶在缺乏整体膜烷烃羟化酶的烷烃降解真细菌中很常见。

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Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases.

机译：在中链烷烃上生长并催化有趣的羟化和环氧化反应的几种菌株不具有完整的膜非血红素铁烷烃羟化酶。使用PCR，我们显示这些菌株中的大多数都具有与CYP153A1和CYP153A6相关的酶，即被表征为烷烃羟化酶的细胞色素P450酶。构建了单个CYP153基因与铁氧还蛋白基因和铁氧还蛋白还原酶基因多顺反子共表达的载体。测试的11种CYP153基因中有7种允许恶臭假单胞菌GPo12重组体在烷烃上生长良好，提供了新克隆的P450确实是烷烃羟化酶的证据。

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van Beilen, Jan B.; Funhoff, Enrico G.; van Loon, Alexander; Just, Andrea; Kaysser, Leo; Bouza, Manuel; Holtackers, René; Röthlisberger, Martina; Li, Zhi; Witholt, Bernard;
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年度 2006
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1. Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases [J] . Jan B. van Beilen, Enrico G. Funhoff, Alexander van Loon, Applied and Environmental Microbiology . 2006,第1期

机译：CYP153家族的细胞色素P450烷烃羟化酶在缺乏整体膜烷烃羟化酶的烷烃降解真细菌中很常见。
2. Alkane Hydroxylase from Acinetobactersp. Strain ADP1 Is Encoded by alkM and Belongs to a New Family of Bacterial Integral-Membrane Hydrocarbon Hydroxylases [J] . Andreas Ratajczak, Walter Gei?d?rfer, Wolfgang Hillen Applied and Environmental Microbiology . 1998,第4期

机译：来自不动杆菌属的烷烃羟化酶。菌株ADP1由alkM编码，属于细菌整合膜碳氢化合物羟化酶的新家族。
3. Alkane Hydroxylase from Acinetobactersp. Strain ADP1 Is Encoded by alkM and Belongs to a New Family of Bacterial Integral-Membrane Hydrocarbon Hydroxylases [J] . Andreas Ratajczak, Walter Gei?d?rfer, Wolfgang Hillen Applied and Environmental Microbiology . 1998,第4期

机译：来自不动杆菌属的烷烃羟化酶。菌株ADP1由alkM编码，属于细菌整合膜碳氢化合物羟化酶的新家族。
4. Cytochrome P450-dependent fatty acid hydroxylases in plants [C] . S.Kandel, V.Sauveplane, A.Olry the Phytochemical Society of Europe Meeting . 2006

机译：细胞色素p450依赖性脂肪酸羟基酶在植物中
5. Expression and regulation of prostaglandin receptors and cytochrome P450c17 hydroxylase in the late gestation ovine placenta. [D] . Fantauzzi, Michelle Rosemary. 2008

机译：妊娠晚期胎盘中前列腺素受体和细胞色素P450c17羟化酶的表达和调控。
6. Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases [O] . Jan B. van Beilen, Enrico G. Funhoff, Alexander van Loon, 2006

机译：CYP153家族的细胞色素P450烷烃羟化酶在缺乏整体膜烷烃羟化酶的烷烃降解真细菌中很常见。
7. Evidence linking the Pseudomonas oleovorans alkane ω-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family [O] . Shanklin John, Whittle Edward 2003

机译：将油假单胞菌烷烃ω-羟化酶，完整的膜二铁酶和脂肪酸脱氢酶家族联系起来的证据
8. Structural and Kinetic Studies of Novel Cytochrome P450 Small-Alkane Hydroxylases. [R] . Arnold, F. H. 2012

机译：新型细胞色素p450小烷烃羟化酶的结构和动力学研究。

Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases†

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